Western Blot:Article Title: USP25 Elevates SHLD2-Mediated DNA Double-Strand Break Repair and Regulates Chemoresponse in Cancer.
Article Snippet: .. In the study, the primary antibodies utilized were as follows: anti-USP25 (Abcam: ab187156, WB:1: 1000; Proteintech: 12199-1-AP, IHC: 1: 250; IF: 1: 100; IP: 1: 100); anti-FAM35A (Novus Biologicals: NBP1-88980, WB: 1: 500); anti-C20orf196 (Novus Biologicals: NBP1-82062, WB: 1: 500); antiCTC-534A2.2 (Novus Biologicals: NBP2-49564, WB: 1: 1000); anti-Mad2L2 (Abcam: ab180579, WB: 1: 1000); anti-γH2AX (Millipore: 05–636, IF: 1: 1000); anti-phospho-RPA32(BETHYL: A300-245A, WB: 1:500); anti-RPA32 (Santa Cruz: sc-56770, IF: 1:500); anti-RAD51 (GENETEX: GTX100469, IF: 1:500); anti-53BP1 (Novus Biologicals: NB100-304, WB: 1:1000, IF: 1:500); anti-BRCA1 (Santa Cruz: sc-6954, IF: 1:250); anti-RIF1(Bethyl: A300-569A, WB: 1:1000); anti-MDC1 (ABclonal: A8358,WB: 1:500); antip-SQ/TQ (ABclonal: AP0933, WB: 1:500); anti-Ub (Santa cruz: sc-8017, WB: 1:1000); anti-GST (ABclonal: AE001, WB: 1:1000); anti-TRIM25 (Proteintech: 12573-1-AP, WB: 1:1000, IHC: 1: 250); anti-WWP2 (Proteintech: 12197-1-AP, WB:1:1000); anti-HECTD3(Proteintech:11487-1-AP, WB:1:1000); anti-NEDD4L (Proteintech:13690-1-AP, WB: 1:1000); antiTubulin (Proteintech: 66031-1-lg, WB: 1:1000); anti-GAPDH (ABclonal: A19056, WB: 1:1000); anti-β-actin (Sigma: A2228, WB: 1: 2000); anti-Flag (Proteintech: 20543-1-AP, WB: 1:1000); anti-HA (ABclonal: AE036, WB: 1: 2000); anti-MYC (Proteintech: 16286-1-AP, WB: 1: 1000); anti-GFP (Abcam: ab290, IF: 1: 1000); anti-H3 (ABclonal: A2348, IF: 1: 1000); anti-K48Ub (ABclonal: A3606, IF: 1: 1000); anti-K63-Ub (CST: 5621S, IF: 1: 500); anti-AID (ABclonal: A16217, WB: 1:1000); PE-Cy7 anti-mouse B220 (BD: 552772, FACS: 1:200); APC anti-mouse CD19 (BD: 550992, FACS: 1:200); FITC anti-mouse IgG3 (BD: 553403, FACS: 1:200); FITC anti-mouse IgE (BD: 553415; FACS: 1:200); FITC anti-mouse CD8 (BD: 553030; FACS: 1:200); PE anti-mouse CD4 (BD: 553048; FACS: 1:200). ..
Immunohistochemistry:Article Title: USP25 Elevates SHLD2-Mediated DNA Double-Strand Break Repair and Regulates Chemoresponse in Cancer.
Article Snippet: .. In the study, the primary antibodies utilized were as follows: anti-USP25 (Abcam: ab187156, WB:1: 1000; Proteintech: 12199-1-AP, IHC: 1: 250; IF: 1: 100; IP: 1: 100); anti-FAM35A (Novus Biologicals: NBP1-88980, WB: 1: 500); anti-C20orf196 (Novus Biologicals: NBP1-82062, WB: 1: 500); antiCTC-534A2.2 (Novus Biologicals: NBP2-49564, WB: 1: 1000); anti-Mad2L2 (Abcam: ab180579, WB: 1: 1000); anti-γH2AX (Millipore: 05–636, IF: 1: 1000); anti-phospho-RPA32(BETHYL: A300-245A, WB: 1:500); anti-RPA32 (Santa Cruz: sc-56770, IF: 1:500); anti-RAD51 (GENETEX: GTX100469, IF: 1:500); anti-53BP1 (Novus Biologicals: NB100-304, WB: 1:1000, IF: 1:500); anti-BRCA1 (Santa Cruz: sc-6954, IF: 1:250); anti-RIF1(Bethyl: A300-569A, WB: 1:1000); anti-MDC1 (ABclonal: A8358,WB: 1:500); antip-SQ/TQ (ABclonal: AP0933, WB: 1:500); anti-Ub (Santa cruz: sc-8017, WB: 1:1000); anti-GST (ABclonal: AE001, WB: 1:1000); anti-TRIM25 (Proteintech: 12573-1-AP, WB: 1:1000, IHC: 1: 250); anti-WWP2 (Proteintech: 12197-1-AP, WB:1:1000); anti-HECTD3(Proteintech:11487-1-AP, WB:1:1000); anti-NEDD4L (Proteintech:13690-1-AP, WB: 1:1000); antiTubulin (Proteintech: 66031-1-lg, WB: 1:1000); anti-GAPDH (ABclonal: A19056, WB: 1:1000); anti-β-actin (Sigma: A2228, WB: 1: 2000); anti-Flag (Proteintech: 20543-1-AP, WB: 1:1000); anti-HA (ABclonal: AE036, WB: 1: 2000); anti-MYC (Proteintech: 16286-1-AP, WB: 1: 1000); anti-GFP (Abcam: ab290, IF: 1: 1000); anti-H3 (ABclonal: A2348, IF: 1: 1000); anti-K48Ub (ABclonal: A3606, IF: 1: 1000); anti-K63-Ub (CST: 5621S, IF: 1: 500); anti-AID (ABclonal: A16217, WB: 1:1000); PE-Cy7 anti-mouse B220 (BD: 552772, FACS: 1:200); APC anti-mouse CD19 (BD: 550992, FACS: 1:200); FITC anti-mouse IgG3 (BD: 553403, FACS: 1:200); FITC anti-mouse IgE (BD: 553415; FACS: 1:200); FITC anti-mouse CD8 (BD: 553030; FACS: 1:200); PE anti-mouse CD4 (BD: 553048; FACS: 1:200). ..
FACS:Article Title: USP25 Elevates SHLD2-Mediated DNA Double-Strand Break Repair and Regulates Chemoresponse in Cancer.
Article Snippet: .. In the study, the primary antibodies utilized were as follows: anti-USP25 (Abcam: ab187156, WB:1: 1000; Proteintech: 12199-1-AP, IHC: 1: 250; IF: 1: 100; IP: 1: 100); anti-FAM35A (Novus Biologicals: NBP1-88980, WB: 1: 500); anti-C20orf196 (Novus Biologicals: NBP1-82062, WB: 1: 500); antiCTC-534A2.2 (Novus Biologicals: NBP2-49564, WB: 1: 1000); anti-Mad2L2 (Abcam: ab180579, WB: 1: 1000); anti-γH2AX (Millipore: 05–636, IF: 1: 1000); anti-phospho-RPA32(BETHYL: A300-245A, WB: 1:500); anti-RPA32 (Santa Cruz: sc-56770, IF: 1:500); anti-RAD51 (GENETEX: GTX100469, IF: 1:500); anti-53BP1 (Novus Biologicals: NB100-304, WB: 1:1000, IF: 1:500); anti-BRCA1 (Santa Cruz: sc-6954, IF: 1:250); anti-RIF1(Bethyl: A300-569A, WB: 1:1000); anti-MDC1 (ABclonal: A8358,WB: 1:500); antip-SQ/TQ (ABclonal: AP0933, WB: 1:500); anti-Ub (Santa cruz: sc-8017, WB: 1:1000); anti-GST (ABclonal: AE001, WB: 1:1000); anti-TRIM25 (Proteintech: 12573-1-AP, WB: 1:1000, IHC: 1: 250); anti-WWP2 (Proteintech: 12197-1-AP, WB:1:1000); anti-HECTD3(Proteintech:11487-1-AP, WB:1:1000); anti-NEDD4L (Proteintech:13690-1-AP, WB: 1:1000); antiTubulin (Proteintech: 66031-1-lg, WB: 1:1000); anti-GAPDH (ABclonal: A19056, WB: 1:1000); anti-β-actin (Sigma: A2228, WB: 1: 2000); anti-Flag (Proteintech: 20543-1-AP, WB: 1:1000); anti-HA (ABclonal: AE036, WB: 1: 2000); anti-MYC (Proteintech: 16286-1-AP, WB: 1: 1000); anti-GFP (Abcam: ab290, IF: 1: 1000); anti-H3 (ABclonal: A2348, IF: 1: 1000); anti-K48Ub (ABclonal: A3606, IF: 1: 1000); anti-K63-Ub (CST: 5621S, IF: 1: 500); anti-AID (ABclonal: A16217, WB: 1:1000); PE-Cy7 anti-mouse B220 (BD: 552772, FACS: 1:200); APC anti-mouse CD19 (BD: 550992, FACS: 1:200); FITC anti-mouse IgG3 (BD: 553403, FACS: 1:200); FITC anti-mouse IgE (BD: 553415; FACS: 1:200); FITC anti-mouse CD8 (BD: 553030; FACS: 1:200); PE anti-mouse CD4 (BD: 553048; FACS: 1:200). ..
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